Molecular Relatedness of Mammalian RNA Tumor Viruses as Deternined by DNA · RNA Hybridization

See allHide authors and affiliations

Science  01 Jun 1973:
Vol. 180, Issue 4089, pp. 972-974
DOI: 10.1126/science.180.4089.972


Each of six mammalian C-type viruses—including two feline leukemia viruses, three murine leukemia viruses, and the human "candidate" virus RD-114—can be distinguished from each other by hybridizing DNA synthesized by viral reverse transcriptase with viral RNA. Characterization of the DNA · RNA hybrids by hydroxylapatite chromatography revealed nucleotide sequence diversity among the viruses, detectable both by the amount of cross-hybridization and by the decreased thermal stability of heterologous hybrids.