Estimation of the Half-Life of a Secretory Protein Message

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Science  08 Jun 1973:
Vol. 180, Issue 4090, pp. 1071-1072
DOI: 10.1126/science.180.4090.1071


Synthesis of a specific secretory protein was followed in the presence of actinomycin D and cordycepin (3'-deoxyadenosine). The apparent half-life of the messenger RNA for this protein is significantly longer in the presence of actinomycin D than in the presence of cordycepin. These results suggest that actinomycin D studies of half-life in specialized cells may be inaccurate.