Temperature-Sensitive Pawns: Conditional Behavioral Mutants of Paramecium aurelia

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Science  15 Jun 1973:
Vol. 180, Issue 4091, pp. 1197-1199
DOI: 10.1126/science.180.4091.1197


"Pawns" are mutants of Paramecium aurelia in which the process of calcium activation during membrane excitation is genetically impaired, with a corresponding loss of avoiding reactions. Mutants are selected that behave normally when grown at 23°C but as pawns at 35°C. The normal excitation can now be disrupted and restored in the same strain at will.