Diisopropylfluorophosphate: Suppression of Ionic Conductance of the Cholinergic Receptor

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Science  31 Aug 1973:
Vol. 181, Issue 4102, pp. 853-856
DOI: 10.1126/science.181.4102.853


When frog sartorius muscles were exposed to diisopropylfluorophosphate, the amplitude and half-decay time of the end-plate current decreased; the half-decay time became almost potential-independent and the equilibrium potential for the end-plate current was more negative than during control conditions. When the excess reagent was removed by washing so that only the phosphorylated acetylcholinesterase remained, the amplitude of the end-plate current was restored, while its half-decay time was markedly increased. These findings reveal that this organophosphate significantly affects the receptor-ionic conductance modulator complex in addition to its well-known anticholinesterase activity.