Gas Chromatographic Assay of Epoxide Hydrase Activity with 3-Methylcholanthrene-11,12-Oxide

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Science  07 Sep 1973:
Vol. 181, Issue 4103, pp. 951-952
DOI: 10.1126/science.181.4103.951


Epoxide hydrase has been measured in rat tissue with 3-methylcholanthrene-11,12-oxide as substrate; diol formation was assayed by gas chromatographic separation of the trimethylsilylated derivative of trans-11,12-dlhydro-11,- 12-dihydroxy-3-methylcholanthrene from the corresponding derivative of the 11 (or 12)-hydroxy-3-methylcholanthrene on 3 percent OV-17, which is formed from the 11,12-oxide during the derivatization. The polycyclic hydrocarbons were extracted initially from the incubation mixture with ethyl acetate. The assay is simple, inexpensive, and sensitive.