Synthesis of RNA-Polyadenylic Acid by Isolated Brain Nuclei

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Science  14 Sep 1973:
Vol. 181, Issue 4104, pp. 1064-1065
DOI: 10.1126/science.181.4104.1064


Nuclei, isolated from mouse brain tissue at various stages of postnatal development and incubated under cell-free conditions, synthesized RNA molecules that were associated with polyadenylic acid [poly(A)]. The RNA synthesized by these nuclei was similar to the poly(A)-associated products described for intact eukaryotic cells. The brain nuclei synthesized a similar proportion of RNA-poly(A) in the presence either of Mg2+ or of Mn2+ with (NH4)2So4. The RNA from neonatal brain nuclei appeared to have a greater proportion of poly(A)-containing RNA than nuclear products obtained from more mature neural tissue.