Gelatin: A Poor Substrate for a Mammalian Collagenase

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Science  05 Oct 1973:
Vol. 182, Issue 4107, pp. 70-71
DOI: 10.1126/science.182.4107.70


A rabbit tumor collagenase was purified more than 5000-fold. In this form it degrades native collagen in helical conformation at 37°C, pH 7.6, into two fragments, but it had little capacity to cleave gelatin, an indication of the importance of higher-order structure of substrate for this enzyme in pure form. It is likely that, in vivo, enzymes other than collagenase degrade gelatin polypeptides produced by primary collagenolysis.