Collagen Synthesis: Localization of Prolyl Hydroxylase in Tendon Cells Detected with Ferritin-Labeled Antibodies

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Science  23 Nov 1973:
Vol. 182, Issue 4114, pp. 825-827
DOI: 10.1126/science.182.4114.825


An improved procedure was employed for linking ferritin to antibodies against prolyl hydroxylase, the enzyme that synthesizes the hydroxyproline in collagen. By electron microscopy, the enzyme was then found to be localized in cisternae of the rough endoplasmic reticulum of embryonic tendon cells; this indicates that hydroxylation of proline occurs while newly synthesized polypeptides are fed into the cisternae.