Neuron Geometry and Circuitry via the Electron Microscope: Intracellular Staining with Osmiophilic Polymer

Science  21 Dec 1973:
Vol. 182, Issue 4118, pp. 1256-1258
DOI: 10.1126/science.182.4118.1256


Intracellularly injected cobalt catalyzes the generation of an osmiumbinding polymer from 3,3'-diaminobenzidine in neurons of Aplysia californica. Excellent structural preservation of nervous tissue is coupled with easy recognition of fine branches of the injected neurons in the electron microscope. This permits detailed structural analysis of synaptology.