Phosphate-Induced Protein Chromatography

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Science  21 Dec 1973:
Vol. 182, Issue 4118, pp. 1268-1270
DOI: 10.1126/science.182.4118.1268


High phosphate concentration is shown to cause a large proportion of the proteins of a cell-free extract of Escherichia coli to bind to agarose columns to which L-valine is attached. With a decreasing concentration gradient of potassium phosphate, the proteins elute in relation to their solubility in concentrated ammonium sulfate. This column technique appears to provide a general tool for the purification of proteins.