Choline Content of Rat Brain

Science  08 Mar 1974:
Vol. 183, Issue 4128, pp. 964-965
DOI: 10.1126/science.183.4128.964


The free choline concentration in the rat brain was found to be 26.3 nanomoles per gram of brain tissue. This value was obtained through use of 6-second microwave irradiation for killing animals and inactivating enzymes, followed by a pyrolysis-gas chromatographic assay procedure. The identities of compounds measured from brain samples were verified by mass spectrometry.