Enzyme Measurements on Single Cells

Science  31 May 1974:
Vol. 184, Issue 4140, pp. 992-994
DOI: 10.1126/science.184.4140.992


A simple and sensitive procedure has been developed for enzymatic assays in single cells and applied to the measurement of β-glucuronidase in single parenchymal cells of liver. Cells deposited in microdroplets under oil were ruptured by freezing and thawing and allowed to react with a fluorogenic substrate. The fluorescence is developed by diffusing an organic base into the droplets and measured in a fluorescence microscope equipped with a photomultiplier.