Thyroid Hormone Action: In vitro Demonstration of Putative Receptors in Isolated Nuclei and Soluble Nuclear Extracts

Science  14 Jun 1974:
Vol. 184, Issue 4142, pp. 1188-1191
DOI: 10.1126/science.184.4142.1188


Saturable binding activities for triiodothyronine were demonstrated in vitro with isolated nuclei and soluble nuclear extracts of rat liver, kidney, and cultured GH1 cells. The binding activity can be extracted from nuclei in soluble form with no significant change in hormone affinity and has properties of a nonhistone protein.