Reports

Cell-Cycle Analysis in 20 Minutes

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Science  21 Jun 1974:
Vol. 184, Issue 4143, pp. 1297-1298
DOI: 10.1126/science.184.4143.1297

Abstract

Mithramycin added to mammalian cells fixed in aqueous ethanol is bound to DNA and fluoresces in direct proportion to the cellular DNA content. Quantitative fluorescence measurement by means of a high-speed flow system instrument provides a rapid method for cell-cycle analysis and for the first time permits continuous monitoring of cell-cycle kinetics during ongoing experiments.