Cell-Cycle Analysis in 20 Minutes

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Science  21 Jun 1974:
Vol. 184, Issue 4143, pp. 1297-1298
DOI: 10.1126/science.184.4143.1297


Mithramycin added to mammalian cells fixed in aqueous ethanol is bound to DNA and fluoresces in direct proportion to the cellular DNA content. Quantitative fluorescence measurement by means of a high-speed flow system instrument provides a rapid method for cell-cycle analysis and for the first time permits continuous monitoring of cell-cycle kinetics during ongoing experiments.