Aldosterone-Induced Protein in Toad Urinary Bladder

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Science  18 Oct 1974:
Vol. 186, Issue 4160, pp. 269-272
DOI: 10.1126/science.186.4160.269


Simultaneous electrophysiological and biochemical experiments demonstrated a specific aldosterone-induced protein in paired urinary hemibladders isolated from the toad Bufo marinus. Whenever aldosterone stinlmulated short-circuit current, aldosterone specifically increased [35S] methionine incorporation into a low-molecular-weight protein (about 12,000). Comparative studies with dexamethasone and insulin and inhibitory studies with spironolactone and actinomycin D suggest mineralocorticoid specificity.