Serological Detection of Mixed Lymphocyte Culture Identity between Cells That Differ by One HL-A Haplotype

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Science  20 Dec 1974:
Vol. 186, Issue 4169, pp. 1124-1126
DOI: 10.1126/science.186.4169.1124


Antibody mediated cell dependent immune lympholysis (ABCIL), an extremely sensitive serological technique for detecting tissue sensitization, was used in a family segregation study. Two serums capable of demonstrating ABCIL were used to identify members of the family who differed by one HL-A haplotype but whose cells did not stimulate in mixed lymphocyte culture. Absorption study of one serum indicated that the ABCIL reaction of that serum was directed against a factor, independent of HL-A, that might be responsible for lymphocyte stimulation in mixed lymphocyte culture. Thus the ABCIL technique may be used to detect lymphocyte-defined gene products.