Microtubule-Macrotubule Transitions: Intermediates after Exposure to the Mitotic Inhibitor Vinblastine

Science  27 Dec 1974:
Vol. 186, Issue 4170, pp. 1219-1221
DOI: 10.1126/science.186.4170.1219


Vinblastine treatment of microtubule protein or intact microtubules assembled in vitro produced bifilar rings and bifilar helices. Suspentsions of rings and helices were demonstrated to bind [3H]colchicine, a diagnostic property of microtubule protein. Macrotubules are suggested to consist of tightly coiled helices formed by longitudinal compacting of loosely coiled protofilament pair intermediates.