Preparation of Native Chromatin and Damage Caused by Shearing

Science  28 Mar 1975:
Vol. 187, Issue 4182, pp. 1203-1206
DOI: 10.1126/science.187.4182.1203


Chromatin prepared by a method involving limited nuclease digestion contains the same repeating structure as chromatin in the nucleus, whereas chromatin prepared by conventional methods involving shear does not.