Reconstitution of Rh (D) antigen activity from human erythrocyte membranes solubilized by deoxycholate

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Science  04 Apr 1975:
Vol. 188, Issue 4183, pp. 66-67
DOI: 10.1126/science.803714


Proteins were selectively solubilized from human erythrocyte membranes with deoxycholate. After ultracentrifugation and preliminary fractionation procedures, the detergent was removed from the soluble membranes by Bio-Beads SM-2 and dialysis against 5 millimolar magnesium chloride. Reaggregation took place with the apparent formation of vesicles which showed serologically specific Rh antigen activity.