Electron spin resonance for detecting polyadenylate tracts in RNA's

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Science  11 Apr 1975:
Vol. 188, Issue 4184, pp. 153-155
DOI: 10.1126/science.163495


Electron spin resonance is used to detect RNA's that contain polyadenylate tracts. The method depends on the ability of RNA's that contain polyadenylate sequences to associate with poly(2'-deoxy-2'-fluoro)uridylic acid, which has been spin-labeled with 4-(2-iodoacetamido)-2,2,6,6-tetramethylpiperidinooxyl. The formation of the hybridization product can be detected by monitoring the decrease in mobility of the spin probe.