Tetrahedral intermediate in a specific alpha-chymotrypsin inhibitor complex detected by laser Raman spectroscopy

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Science  01 Aug 1975:
Vol. 189, Issue 4200, pp. 384-386
DOI: 10.1126/science.238285


Laser Raman spectroscopy indicates that inhibition of alpha-chymotrypsin by 2-phenylethaneboronic acid occurs in two steps: (i) the formation of a loosely bound complex, in which the boron remains in a trigonal configuration; and (ii) reaction of the boron with a functional group in the catalytic site of the enzyme, resulting in a reversible, stable tetrahedral adduct.