Guanidinium-CsCl density gradients for isopycnic analysis of nucleic acids

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Science  07 Nov 1975:
Vol. 190, Issue 4214, pp. 584-586
DOI: 10.1126/science.1188358


The addition of guanidinium chloride to CsCl gradients lowers the apparent density of RNA, DNA, and hybrid polymers in such a way that all three can be banded, fractionated, and analyzed in one gradient with essentially no damage to their chemical integrity or to their biological activity.