Molecular vehicle properties of the broad host range plasmid RK2

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Science  19 Dec 1975:
Vol. 190, Issue 4220, pp. 1226-1228
DOI: 10.1126/science.1060178


The pladmid RK2 is stably maintained in a broad range of gram-negative bacteria. The RK2 DNA has a single Eco RI restriction site. The insertion of a DNA fragment into this site does not interfere with either plasmid maintenance or self-transmissibility. Because RK2 has a broad host range, it should be useful for the construction in vitro of hybrid plasmid molecules capable of being established by conjugal transfer or transformation into many genera of gram-negative organisms.