Cytoplasmic microtubule organizing centers isolated from Polytomella agilis

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Science  16 Jan 1976:
Vol. 191, Issue 4223, pp. 188-191
DOI: 10.1126/science.1246607


Basal body rootlets in Polytomella function as organizing centers for cytoplasmic microtbules in vivo. A method is described to isolate intact basal body-rootlet complexes. The integrity of the isolated complexes is confirmed by electron microscopy, and the rootlets are shown to be competent as initiation sites for the in vitro polymerization of brain microtubule protein.