Reports

Measurement of membrane protein lateral diffusion in single cells

Science  06 Feb 1976:
Vol. 191, Issue 4226, pp. 466-468
DOI: 10.1126/science.1246629

Abstract

Fluorescence rapidly returns to spots bleached by a laser beam in the continuous fluorescence of cultured cells labeled on the surface with fluorescein isothiocyanate. The rate of recovery of fluorescence after bleaching can be interpreted as a measure of the lateral diffusion of integral membrane proteins labeled with fluorescein.

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