Proviral DNA of Moloney leukemia virus: purification and visualization

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Science  13 Feb 1976:
Vol. 191, Issue 4227, pp. 569-571
DOI: 10.1126/science.1251192


Closed-circular proviral DNA of Moloney leukemia virus has been purified from a 10(7) excess of cellular and mitochondrial DNA. The DNA can be visualized in the electron microscope and has the contour length of a molecule with a moleculecular weight of about 5.5 + 10(6). Electron microscopic observation of a hybrid between viral RNA and this circular DNA confirms the viral origin of this molecule.