Shoot Initiation from Carnation Shoot Apices Frozen to –196°C

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Science  19 Mar 1976:
Vol. 191, Issue 4232, pp. 1178-1179
DOI: 10.1126/science.191.4232.1178


Aseptically excised shoot apices from Dianthus caryophyllus were frozen to –196°C and subsequently thawed to room temperature. Survival rates as high as 33 percent, determined by callus formation, growth, or chlorophyll formation, are reported; plant formation has been observed.