Human pancreatic islets in culture: effects of supplementing the medium with homologous and heterologous serum

See allHide authors and affiliations

Science  04 Jun 1976:
Vol. 192, Issue 4243, pp. 1014-1016
DOI: 10.1126/science.775637


Islets of Langerhans, isolated from the human fetal pancreas and cultured in media supplemented with serums from human umbilical cord or adult donors, remained intact, free-floating, and functional for 2 to 5 months. Substitution of fetal calf serum for the human serum usually resulted in attachment of islets, monolayer formation, and relatively early (3 weeks) functional arrest.