Reports

Human pancreatic islets in culture: effects of supplementing the medium with homologous and heterologous serum

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Science  04 Jun 1976:
Vol. 192, Issue 4243, pp. 1014-1016
DOI: 10.1126/science.775637

Abstract

Islets of Langerhans, isolated from the human fetal pancreas and cultured in media supplemented with serums from human umbilical cord or adult donors, remained intact, free-floating, and functional for 2 to 5 months. Substitution of fetal calf serum for the human serum usually resulted in attachment of islets, monolayer formation, and relatively early (3 weeks) functional arrest.

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