(+)-alpha-(N-1-phenethyl) urea stereospecifically inhibits Ca2+ - but not ADP-stimulated mitochondrial respiration

Science  02 Jul 1976:
Vol. 193, Issue 4247, pp. 66-68
DOI: 10.1126/science.935857


The (+) isomer of alpha-(N-1-phenethyl) urea is a moderately potent inhibitor of Ca2+ -stimulated mitochondrial respiration of 45Ca2+ uptake (50 percent inhibition at 0.18 mM) while having no effect on adenosine diphosphate-stimulated respiration; the (-) isomer is without effect up to 4 mM. beta-Phenethylurea does not inhibit with either stimulus. The data support the involvement of a Ca2+ -specific protein in energized mitochondrial Ca2+ uptake.