Interkingdom fusion between human (HeLa) cells and tobacco hybrid (GGLL) protoplasts

See allHide authors and affiliations

Science  30 Jul 1976:
Vol. 193, Issue 4251, pp. 401-403
DOI: 10.1126/science.935875


The fusion of human HeLa cells with tobacco protoplasts has been accomplished with the use of polyethylene glycol. The sequence from heterocellular adherence to heterokaryon formation has been followed with light microscopy and confirmed by autoradiographs of heterokaryons containing unlabeled tobacco nuclei and tritium-labeled HeLa nuclei. The HeLa nucleus retained its integrity in the tobacco cytoplasm up to 6 days after fusion.