Reports

Cholera toxin-peroxidase: changes in surface labeling of glioblastoma cells with increased time in tissue culture

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Science  13 Aug 1976:
Vol. 193, Issue 4253, pp. 588-590
DOI: 10.1126/science.959818

Abstract

Cholera toxin coupled to peroxidase yielded a highly specific ultrastructural marker of plasma membrane monosialogangliosides. Studies with cultures of brain and brain tumors suggested that long-term culture of tissue in monolayers results in eventual loss of surface monosialogangliosides.