Histones and G banding of chromosomes

See allHide authors and affiliations

Science  13 Aug 1976:
Vol. 193, Issue 4253, pp. 599-602
DOI: 10.1126/science.959822


Polylysine, polyarginine, and histones H1, H2A, H2B, and H3 inhibit Giemsa staining and chromosome banding by binding to DNA and preventing side stacking of the positively charged thiazine dyes to the negatively charged phosphate groups on DNA. This is a nonspecific effect and does not of itself provide evidence for a role of histones in G banding. The question of whether histones are involved in chromosome banding is reviewed.