Reports

Novel screening procedure for recombinant plasmids

See allHide authors and affiliations

Science  28 Jan 1977:
Vol. 195, Issue 4276, pp. 391-393
DOI: 10.1126/science.318763

Abstract

Lysed bacterial colonies containing potential recombinant plasmids were mixed with molten agar and sealed into slots of an agarose gel. After electrophoresis overnight, the gel was stained with ethidium bromide, which clearly reveals recombinant plasmids. Xenopus laevis ribosomal DNA and histone DNA of Psammechinus miliaris were ligated into pCRI plasmids and screened by this method.