Plasmid detection and sizing in single colony lysates

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Science  28 Jan 1977:
Vol. 195, Issue 4276, pp. 393-394
DOI: 10.1126/science.318764


A simple and contained procedure for the rapid assay of the presence and size of plasmids similar to Col El is described. Bacteria are picked from an agar plate with a toothpick, lysed with dodecyl sulfate and heat, and placed directly on an agarose gel for electrophoresis.