Liposome-cell interaction: transfer and intracellular release of a trapped fluorescent marker

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Science  04 Feb 1977:
Vol. 195, Issue 4277, pp. 489-492
DOI: 10.1126/science.835007


When small, unilamellar lipid vesicles containing a high concentration of the fluorescent dye 6-carboxyfluorescein are incubated with either frog retinas or human lymphocytes, fluroescence distributes widely throughout each cell. Since "self-quenching" largely prevents the dye from fluorescing as long as it remains sequestered in vesicles, it is clear that a considerable amount of dye is released from the vesicles and diluted into the much larger volume of the cell.