Calcium-induced displacement of membrane-associated particles upon aggregation of chromaffin granules

See allHide authors and affiliations

Science  04 Feb 1977:
Vol. 195, Issue 4277, pp. 495-497
DOI: 10.1126/science.835010


Isolated chromaffin granules incubated in 10 millimolar calcium chloride aggregated, forming contact sites with a pentalaminar membrane structure. These circular attachment sites were free of membrane-associated particles, which accumulated at the periphery. Incubation in 20 millimolar ethylenediaminetetraacetic acid reversed these changes, which are regarded as initial events in the membrane fusion reaction.