Superhelix densities of circular DNA's: a generalized equation for their determination by the bouyant method

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Science  04 Mar 1977:
Vol. 195, Issue 4281, pp. 891
DOI: 10.1126/science.190680


Equations for the measurement of the supertwisting of circular DNA's from banding positions in buoyant density gradients containing intercalating dyes have required the use of SV40 DNA isolated from virions as a reference DNA. These equations are modified to allow the use of any closed circular DNA of known superhelix density as a reference DNA.