Reports

Chemical synthesis of restriction enzyme recognition sites useful for cloning

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Science  08 Apr 1977:
Vol. 196, Issue 4286, pp. 177-180
DOI: 10.1126/science.847463

Abstract

By a triester chemical synthesis method, three decameric DNA's have been made; these act as substrates for several restriction endonucleases, including Eco RI, Bam I, and Hind III. These homogenous decamers form duplexes that can be efficiently blunt-end ligated to themselves or to other DNA molecules by the action of T4 DNA ligase and thus are useful tools for molecular cloning experiments.