Nucleotide sequences from a rabbit alpha globin gene inserted in a chimeric plasmid

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Science  08 Apr 1977:
Vol. 196, Issue 4286, pp. 192-195
DOI: 10.1126/science.847466


Rabbit alpha globin gene copies have been made, using reverse transcriptase and DNA polymerase I, and cloned in bacterial plasmids. Plasmid pHb72 has been shown to contain the alpha gene sequence by restriction enzyme analysis and nucleotide sequencing studies, and therefore has been approved for propagation under P2 plus EK1 conditions by the National REcombinant DNA Committee.