Cloned ribosomal RNA genes from chloroplasts of Euglena gracilis

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Science  08 Apr 1977:
Vol. 196, Issue 4286, pp. 202-205
DOI: 10.1126/science.403604


Fragments of Euglena chloroplast DNA generated by endonuclease R-Eco RI were separated by agarose-gel electrophoresis into 24 distinct bands. At least five fragments contain sequences complementary to chloroplast ribosomal RNA, Most of the Eco RI fragments have been cloned in a plasmid of Escherichia coli. Three of the cloned fragments were shown to contain chloroplast ribosomal RNA sequences by DNA-RNA hybridization.