Tris buffer attenuates acetylcholine responses in Aplysia neurons

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Science  22 Apr 1977:
Vol. 196, Issue 4288, pp. 440-441
DOI: 10.1126/science.15317


The commonly used buffering agent tris(hydroxymethyl)methylamine (tris) antagonizes the action of iontophoretically applied acetylcholine on neurons of Aplysia californica. Concentrations of 5 to 10 millimolar tris markedly reduced both excitatory and inhibitory responses.