Reports

Mechanism of carbon isotope fractionation associated with lipid synthesis

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Science  15 Jul 1977:
Vol. 197, Issue 4300, pp. 261-263
DOI: 10.1126/science.327543

Abstract

The low carbon-13/carbon-12 ratio of lipids is shown to result from isotopic fractionation during the oxidation of pyruvate to acetyl coenzyme A. In vitro analysis of the kinetic isotope effects of this reaction indicates that there will be a large, temperature-dependent difference in the carbon-13/carbon-12 ratio between the methyl and carbonyl carbon atoms of acetyl coenzyme A and between those carbon atoms of lipid components which derive from them.

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