Secondary structure of histones and DNA in chromatin

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Science  22 Jul 1977:
Vol. 197, Issue 4301, pp. 385-388
DOI: 10.1126/science.560060


Laser Raman spectroscopy indicates that the inner histones which are bound to DNA in chromatin or in isolated nu bodies are similar in conformation to the inner histones which are dissociated from DNA in high-salt solutions. This structure contains, on the average, 51+/-5% alpha-helix and no substantial beta-sheet conformation. It is proposed that the protein core of the nu body has a high alpha-helix content.