Double minute chromosomes and the homogeneously staining regions in chromosomes of a human neuroblastoma cell line

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Science  18 Nov 1977:
Vol. 198, Issue 4318, pp. 739-741
DOI: 10.1126/science.71759


Four human neuroblastoma cell lines were studied by chromosome banding techniques. All of the lines contained a marker chromosome with a long nonbanding homogeneously staining region (HSR). The HSR-containing chromosome differed in each line. One line contained two classes of cells: one with an HST marker chromosome and the other with double minute chromosomes. Each cell had one of these abnormalities; no cell had both. The presence of two additional chromosomal markers in all cells of this line indicates a common origin. These observations suggest that the double minute chromosomes are derived from the HSR.