Genetic structure of the replication origin of bacteriophage lambda

Science  09 Dec 1977:
Vol. 198, Issue 4321, pp. 1046-1051
DOI: 10.1126/science.929186


A fragment of bacteriophage lambda DNA produced by the restriction endonuclease Eco RI and extending from the immunity region to a point inside gene O is found to have a fully functional origin of replication. Seven ori- mutations of lambda cluster in a small region just to the left of the Eco RI cleavage site which defines the right end of this fragment. These mutations lie within gene O.