Perfusion preservation of hearts for 6 to 9 days at room temperature

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Science  20 Jan 1978:
Vol. 199, Issue 4326, pp. 299-301
DOI: 10.1126/science.619456


The combination of a defined medium with single-pass perfusion has made possible long-term maintenance of beating rat hearts at 22 degrees C in vitro. The 6- to 9-day survival period appears to be the longest so far reported for hearts. This method provides a stable system which should be useful for investigating the role of single factors in myocardial preservation and evaluating the effects of exposure to pharmacological and toxicological agents.