Bone cells: a serum-free medium supports proliferation in primary culture

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Science  03 Feb 1978:
Vol. 199, Issue 4328, pp. 542-544
DOI: 10.1126/science.564080


Bone-cells isolated from embryonic rat calvaria increase in number two-to threefold when cultured at high, but not at low, population densities in a serum-free medium that contains albumin. Cultured cells respond to parathyroid hormone and exhibit a marked rise in alkaline phosphatase activity during proliferation, which suggests the progressive differentiation or preferential growth of osteoblast-like cells.