Membrane enzymes: artifacts in Arrhenius plots due to temperature dependence of substrate-binding affinity

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Science  24 Feb 1978:
Vol. 199, Issue 4331, pp. 902-904
DOI: 10.1126/science.146257


For the membrane sodium-stimulated magnesium-adenosinetriphosphatase of Acholeplasma laidlawii B both the Vmax and Km values in the Michaelis equation very strongly with temperature. Simulations of Arrhenius plots show that an enzyme with a temperature-dependent Km can yield a variety of Arrhenius plot artifacts, most notably erroneous "breaks," if activity is assayed at a fixed substrate concentration.