Production of antibody to tetanus toxoid by continuous human lymphoblastoid cell lines

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Science  31 Mar 1978:
Vol. 199, Issue 4336, pp. 1439-1441
DOI: 10.1126/science.204013


Peripheral lymphocytes from human volunteers boosted with tetanus toxoid were cultured after in vitro infection with Epstein-Barr virus. Forty-four continuous lymphoblastoid lines were established which continued to secrete human gamma globulin; seven of these secreted antibody to tetanus toxoid. Subcultures derived from limiting dilution experiments continued to secrete the antibody. Some of these antibody-secreting cells have been in continuous culture for more than 6 months.