Collagenase immunolocalization in cultures of rheumatoid synovial cells

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Science  19 May 1978:
Vol. 200, Issue 4343, pp. 773-775
DOI: 10.1126/science.205952


Cultures of rheumatoid synovial cells that have been enzymatically dissociated and are adherent to a culture vessel are morphologically heterogeneous. When these cells are cultured on a collagenous substrate for 2 to 6 days at 37 degrees C in serum-free medium, they produce collagenase. A monospecific antibody to human collagenase has localized the enzyme extracellularly around cytoplasmic extensions of dendritic cells and intracellularly within a few macrophage-like and fibroblast-like cells.